The use of cytotoxic drugs in cancer therapies is increasing annually. 5-Flourouracil (5-FU) is one of the most widely used antineoplastic drugs in the world and the threshold value toxicity of this drug is 94 ng/L. This research applied gold nanoparticles (GNPs) to remove 5-FU through adsorption. Concentrations were determined experimentally by UV-Vis spectroscopy. Effect of Several parameters like; contact time, temperature, pH, effect of 5-FU concentration and dosage of Gold nanoparticles were tested by adsorption experiments. The equilibrium data corresponded better to Sips than Langmuir and Freundlich isotherms. The removal efficiency of 5-FU adsorbed by GNPs was more than 85% at environmental conditions (pH= [7-9], concentration of 5-FU=5mg/L, and temperature=298 K). The kinetic data showed that the adsorption followed pseudo second-order kinetic model. The use of GNPs to adsorb 5-FU was effective process since it was easy to reuse and recover GNPs using 0.1M HCL solution.