The motility, sperm membrane and acrosome integrity during post–thawing incubation is an indication of the usability of the semen. The maintenance of higher motility of sperm during post–thawing incubation simultaneously with the maintenance of intact cell membrane and acrosome integrity reflects a greater likelihood of their survival in the female genital tract to undergo capacitation and fertilize ova or even to be used in in vitro embryo production. The aim of this study was to elucidate the protective ability of royal jelly on motility, spermatozoa plasma membrane and integrity of acrosome during 4 hours of incubation of post-thawed ram semen in thawing media contains different concentrations of RJ (0.5, 1.0 and 2.0%). The post-thaw results revealed that 0.5% RJ supplementation to incubation media significantly improved (P< 0.05) total and progressive motility after the second hour of incubation compared to 0, 1 or 2% RJ groups; however, a non-significant difference (P> 0.05) was obtained regarding the sperm acrosome integrity across all the treatment groups. In conclusion, the structural and functional parameters of sperm during -thawing can be enhanced by supplementing the RJ in post-thawing media before used especially in IVF process and a dose of 0.5% RJ provide the best post-thaw sperm quality.