A study was conducted to evaluate residues of imidazolinone (IMI) in soil. Samples were taken from three Clearfield® rice fields as IMI which have been used for six years. IMI herbicides (imazapic/imazapyr) were widely used in Clearfield® rice soils. To date, few studies are available on the residues of these herbicides, especially in the context of Malaysian soil. Therefore, for this purpose, high performance liquid chromatography (HPLC) with UV detection was performed using a Zorbax stable bond C18 (4.6 × 250 mm, 5 μm) column, with two mobile phases. The average percentage recovery for imazapyr and imazapic varied from 76%-107% and 71-77%, with 0.1-5 μg/ml fortification level, respectively. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.05 and 4.09 for imazapic and 0.171 and 0.511 μg/ml for imazapyr respectively, in the top 15 cm. In the extracted soil sample, it was 0.19 μg/ml for imazapic and 0.04 μg/ml for imazapyr, respectively. Based on this study, a pre-harvest period of 40-60 day is suggested for rice crops after IMI application. © 2017, ALÖKI Kft., Budapest, Hungary.