Midwives and nurses should use the standard precautions as the basic level of infection control precautions when delivering care to all patients, regardless of their presumed infection status. Therefore midwifes and nurses should have sound knowledge and compliance with standard precaution. Aim of the study: The study aimed to assess the level of the compliance of standard precautions among the midwives and nurses in the Palestinian Hospitals. Method: A cross sectional study was conducted from May to June 2015 on 81 midwives and nurses from Palestinian hospitals. The data were collected from labor rooms and postpartum departments of Palestinian hospitals. Data were collected using pretested questionnaire on 81 midwives and nurses selected by convenience sample. Results: The current study showed that the average of standard precautions knowledge level and compliance are 74.6% and 83.8% respectively. There are an association between age, education, work experience, and compliance with standard precautions at p < 0.05 (0.000, 0.031, and 0.043) respectively. At the same time no significant association between training courses and compliance to standard precautions at p < 0.05 (0.191). Conclusion: The midwives and nurses in the current study for both knowledge and compliance have high level regarding standard precautions. There is an association between age, education, work experience, and compliance with standard precautions. Recommendations: Knowledge of midwives and nurses should be updated; the importance of latest evidence-based practices of infection control in continuing education/training program should be emphasized; and training programs for newly midwives and nurses about standard precaution and at regular intervals should be provided.