Introduction:Obesity is an epidemic problem worldwide. Palestine is not an exception, the prevalence of obesity is 4 times among women and 2 times among men higher than the prevalence observed worldwide. It is associated with low-grade inflammation originates mainly from adipose tissue, which is implicated in the pathogenesis of type-2 diabetes and cardiovascular diseases. Strong evidence indicates that chronically elevated systemic low-grade lipopolysaccharide (LPS), elicits low-grade inflammation. However, evidence on LPS effect on pro-inflammatory adipokines, such as leptin, is scarce and it has never been investigated ex vivoin human subcutaneous adipose tissue (SAT). therefore we aim to investigate effect of LPS on leptin and TNF-α release from human SAT.
Materials and methods:SAT explants were obtained from 12 patients (50% males) andwere treated with/without LPS for 2 and 20 hours. Protein concentration was determined by ELISA.
Results:the average of BMI and age was 28.6 Kg/m2and 58.6 years old respectively. LPS induced significantly (~3X, p<0.0001) the secretion of TNF-α from SAT and it was not associated with age or BMI. Leptin release was inhibited slightly (~20%), but significantly. A further analysis revealed the association between leptin response and BMI (R=0.8) and it appeared that leptin release was significantly inhibited by 50% in SAT from lean patients.
Conclusion:In this study we found for the first time that LPS suppresses the release of leptin hormone from human SAT obtained from lean. Our findings provide extra evidence and confirm earlier studies regarding the role of LPS in low-grade inflammation. Further investigations are essential to identify factors, e.g. pharmaceutical compounds, to prevent LPS passage through intestinal barrier to prevent the development of obesity and its associated chronic-diseases.