The emerging drug resistance bacteria increased the demand on the discovery of antibiotics from natural
sources. This research was aimed to study the antibacterial reactivity; as well as the phytochemicals, of the wild type of
Cyclamen persicum, using nine different extraction methods where four solvents (Methanol, Ethanol, Hexane; and
Water) were involved with varied extraction periods ranged from 2 up to 10 hours. The antibacterial activity of crude
methanol extract (CME) was found as the best method of extraction, with particular emphasis on the method with
prolonged extraction time of (10 hrs). The antibacterial activities of produced CME were determined by using agar
diffusion method against two of gram-positive bacteria and two gram-negative ones. The CME treated Mueller-Hinton-
Agar plates, were exhibited antibacterial effects against the gram-positive bacteria (Staphylococcus aureus and Bacillus
subtilis) by showing of inhibition zone after overnight incubation, while nothing was noticed on those of gram negative
ones (Pseudomonas aeruginosa and Escherichia coli). These results that proved the antibacterial activity of the
Cyclamen persicum tubers were positively tested the Saponin glycosides from plant. In addition to that, methanol solvent
could be the useful method for extractions of Cyclamen and can be used in any developing drugs against pathogenic
gram positive bacteria.

Title

Pakistan Journal of Pharmaceutical Sciences

Publisher

University of Karachi

Publisher Country

Pakistan

Indexing

Thomson Reuters

Impact Factor

0.825

Publication Type

Prtinted only

Volume

31

Year

2018

Pages

187-192