Visual and Musculoskeletal Disorders (VMSD) are one of the occupational diseases prevalent among office workers in the globe. Many studies have been devoted to examine such disorders in developed countries. However, few studies have recently been initiated in developing countries. Among them is this study which aims to assess the prevalence of VMSDs among office workers in Palestine. To this end, a self-reporting questionnaire was designed and distributed to 501 respondents from office workers working in public and private organizations in West Bank, Palestine. The collected data were statistically analyzed performing, ANOVA and Multiple Linear Regression. The results showed that about 62.5% of the sampled workers suffered from the development of at least one VMSD symptom, and about 17% indicated they have one permanent symptom. Besides, gender and age of office workers showed significant statistical differences in the prevalence of VMSD’s symptoms. The results of the MLR showed significance in modeling the VMSD’s symptoms prevalence. The findings of this study could help the relevant stakeholders in taking engineering and administrative interventions to alleviate the adverse effects of VMSD on office workers’ occupational health, safety and job performance.