This experiment was conducted to investigate the effect of equine chorionic gonadotropin (eCG) levels on estrus response, onset and duration of estrus, lambing rate and litter size in Assaf ewes. A total of 80Assaf ewes were used in the experiment which was conducted during April, 2016, a month that is considered as non-breeding period in Palestine. Ewes were treated with intravaginal sponges containing 60 mg medroxyprogesterone acetate (MAP). Following withdrawal of sponges at day 14, 30ewes from each treatment groups were injected intramuscularly with 300 or 600 IU (eCG), while the control group (n=20) receive nothing. The results showed that level of eCG had no significant effects on the tested parameters. This finding indicated that low level of eCG can be successfully applied for estrus synchronization in Assaf breed out of the breeding season. Results showed that using sponge followed by a 300 IU dose of eCG could induce estrus successfully with low cost comparing to application of high doses of eCG.