Bioactivity of Camelus dromedarius Whey in Combination with Rosmarinus officinalis Extracts
Publication Type
Conference Paper


Bioactive ingredients of camel milk and plant extracts have gained significant attention of scientists for their medicinal values. Therefore, the present study was performed to evaluate the antibacterial potential of camel whey and Rosmarinus officinalis extracts. In addition to their effect on the activity of Glutathione-S- transferases (GSTs) enzymes.



Whey proteins were prepared by casein precipitation from skimmed milk sample using rennin.  Protein contents of whey were fractionated by SDS-PAGE. Aqueous and ethanol extracts from R. officinalis were prepared to a final concentration 200 mg/ml and 1 mg/ml. The antibacterial activity of all samples and combinations was tested against Staphylococcus aureus and Escherichia coli using disk diffusion method. For further antibacterial investigation, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured by micro-broth dilution method. Furthermore,  GSTs enzymes were extracted and purified from sheep liver using ammonium sulfate precipitation, gel filtration and affinity column chromatography. The effect of camel whey and plant extracts on GSTs was studied by spectrophotometric method using 1-chloro-2,4-dinitro -benzene (CDNB) as substrate.



The obtained results showed that ethanol extract was the most effective when compared to the aqueous extract and whey proteins. Synergistic antibacterial activity was observed for whey and ethanol extract against E. coli with MBC value equal to 0.39 mg/ml. In the contrary, whey combination to aqueous extract showed antagonistic activity. Moreover, results revealed that all samples with 1 mg/ml concentration increased the activity of GSTs. The recorded GSTs activity was higher after combination of whey to both extracts. The best results obtained after combination of ethanol extract to whey proteins.



The obtained results form a platform for further bioactivity studies using different combinations between camel whey and other plant extracts. In addition to that,  active ingredients and their biological combinations effect should be also studied.


Conference Title
The 10th International Palestinian Conference of Laboratory Medicine – The 15th Arab Conference of Clinical Biology
Conference Country
Conference Date
April 18, 2018 - April 21, 2018
Conference Sponsor
نقابة الطب المخبري الفلسطينية
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