Ghaleb Adwan, Kamel Adwan, Naser Jarrar and Alaa Amleh
Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine.
e-mail: [email protected]
Background: Resistance to antimicrobial agents is considered one of the major problem worldwide, especially among nosocomial pathogens. Methicillin resistant S. aureus (MRSA) infections are of special concern due to there are few therapeutic options. In addition, MRSA increases hospital costs and might lead to significantly increased morbidity and mortality in patients as compared to methicillin sensitive S. aureus infections. Objectives: This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes and to investigate the prevalence of macrolide, lincosamide, streptogramin, aminoglycoside and tetracyclin resistance genes among MRSA isolates. Materials and methods: A total of 55 clinical MRSA isolates were included in this study, conventional susceptibility testing (antibiotic (disk diffusion method and broth microdilution assay) has been done and confirmed by multiplex PCR technique. Results: Our results showed that there was no discordance between conventional susceptibility testing and gene detection by multiplex PCR assay. The prevalence of ermA, ermC, tetK, tetM, aacA-aphD, vatA, vatB and vatC gene among MRSA isolates was 30.9%, 74.5%, 76.4%, 16.4%, 74.5, 1.8%, 0% and 5.5%, respectively. These MRSA strains belonged to SCCmec types II, III, IVa and V. Conclusion: Rapid and reliable method for antibiotic susceptibility is important to determine the appropriate therapy decision. Multiplex PCR can be used for confirmation of the results obtained by disk diffusion method or could be used as an alternative diagnostic method in the routine diagnosis for rapid, sensitive, and specific detection of MRSA associated antibiotic resistance genes