Detection of bacterial pathogens in surgical site infections and their antibiotic sensitivity profile
Publication Type
Original research

Surgical site infections considered as a major problem in health care centers, resulting in extended length of stay,
substantial associated morbidity and mortality, and high excess hospital cost. Thirty wound swabs were collected
from patients who had developed postoperative wound infections at Rafidia Hospital-Nablus, Palestine. Bacterial
isolates were identified according to standard microbiological methods. Antibiotics susceptibility test was applied
for all isolated bacterial species. ERIC-PCR was carried out to determine the identity between isolated clones. The
results of this research showed that the prevalence of pathogens among surgical site infections was 56.7%, 30%,
6.7%, 3.3% and 3.3% for E. coli, S. aureus, Klebsiella sp., Enterobacter sp., and Acinatobacter sp., respectively. E.
coli isolates showed high resistance against Nalidixic acid (88.2%), Trimethoprim/Sulfamethoxazole (76.5%),
Tetracycline (70.6%), Norfloxacin (64.7%) and Ciprofloxacin (58.5%). S. aureus showed high resistance against
Nalidixic acid (88.9%), Norfloxacin (77.8%), Amoxycillin/clavulanic acid (77.8%), Kanamycin (66.7%) and
Ciprofloxacin (55.6%). Methicillin resistant S. aureus (MRSA) accounted for 33.3% of a total of S. aureus isolates.
Resistant to 3 or more antibiotics were detected in 94.1% (16/17) and 77.8% (7/9) of E. coli and S. aureus isolates,
respectively. ERIC-PCR typing E. coli and S. aureus isolates showed that each was consisted of 4 ERIC-PCR
clusters at a 50% similarity level. Indistinguishable and closely related strains were detected for both
microorganisms. Results of this study might be important in provoking awareness to postoperative wound infections
and further studies are needed to identify other pathogens responsible for SSIs and the source of infections. Using
effective antibiotic policy will restrict further spread of postoperative wound infections.

International Journal of Medical Research & Health Sciences
International Journal of Medical Research & Health Sciences
Publisher Country
Publication Type
Online only