The Effect of Colchicine on Adventitious Shoot Regeneration from Cultured Leaf Explants of Petunia hybrida
Publication Type
Original research

Aims: The effect of various concentrations of colchicine on adventitious shoot
regeneration of two Petunia hybrid cultivars was studied.
Study Design: All collected data were analyzed using the SAS software. One-way
analysis of variance of the treatments was conducted for each experiment followed by
means separation using the least significant difference (LSD) at 5% probability level.
Place and Duration of the Study: The study was conducted during January 2012 to
December 2012 at the Faculty of Agriculture, An-Najah National University, Palestine.
Methodology: In vitro leaf explants of both ‘Daddy Blue’ and ‘Dreams White’ cultivars
were used. Before incubation on the shoot regeneration medium, the leaves were cut
transversely into two pieces and were soaked for three hours in different concentrations of
colchicine solution (0.025, 0.05 and 0.1% w/v), colchicines application was done at zero
incubation time and after 1, 2 or 3 weeks of culture incubation. Wet control was used by
soaking explants in sterile distilled water.
Results: The ability to form shoots was highly reduced with colchicine application.
Significant reduction in the regeneration frequency was observed with the two cultivars.
Shoot regeneration percentage was reduced from 78.3% and 90.0 % without colchicine to
13.3% and 5.2% when colchicine was added after two weeks of incubation at 0.025% in

‘Daddy Blue’ and ‘Dreams White’, respectively. The colchicine treatments at different
intervals gave significantly lower regeneration percentages than the control in both
cultivars. No regeneration was observed when colchicine was added after three weeks of
incubation, and at 0.1% colchicine level at all incubation treatments. A similar trend of
average number of shoots produced was observed.
Conclusion: This study showed a high reduction in regeneration percentage due to
colchicine application with slight difference in response between the two tested cultivars;
therefore, other evaluation methods are needed in the future.

British Biotechnology Journal, 4(5): 531-540, 2014
Publisher Country
Publication Type
Both (Printed and Online)