Generation of Mouse Monoclonal Antibodies Against Camelus Dromedarius IgG3 Heavy Chain Antibodies
Publication Type
Conference Paper

Camelidae have a unique humoral immune response, as they are the only known mammals that possess two different types of IgG antibodies. One type of these antibodies is IgG1 conventional antibodies which is composed of two heavy chains and two light chains. The other type is unusual as these antibodies lack the light chains and so called heavy chain antibodies (Hamers-Casterman et al., 1993). Scientists found that these antibodies acquired unique characteristics. They are of small size, so they can enter easily into cells and tissues (Cortez-Retamozo et al., 2004). Heavy chain antibodies are highly stable (Dumoulin et al., 2002), so they can be used in different applications that depends on their stability. For isotyping of camel IgGs, it is easier to use monoclonal antibodies against each isotype. Daley et al. (2005) produced isotype-specific mouse monoclonal antibodies for camel IgG1. Azwai et al., (1995) generated monoclonal antibodies specific for camel IgG and IgM Heavy and light chains. These monoclonal antibodies were only reactive with IgG1 isotype and no IgG2 and IgG3 specific monoclonal antibodies were generated. Here we describe the generation of monoclonal antibodies producing hybridoma against Camelus dromedarius IgG3. The hypridoma production was completely successful as 25% of these hybridomas reacted specifically with IgG3 isotype in Camelus dromedarius sera and milk. No cross reactivity between IgG3 and other isotypes (IgG1 and IgG2) was found. Two different monoclonal antibodies isotypes were produced which are IgG1 and IgG3. Monoclonal antibodies (clone A6G11) was used as detection system for studying of thermal stability of camel IgG3. Interestingly, our anti-camel IgG3 antibodies were able to detect the presence of IgG3 in camel urine.

Conference Title
The 2nd Conference on Biotechnology Research and Application in Palestine (An-Najah University, Nablus, September, 26-27, 2010)
Conference Country
Conference Date
Sept. 26, 2010 - Sept. 26, 2010
Conference Sponsor
An-Najah National University