A total of 11 durum wheat varieties were grown in the green house to study the morphological differences: Six landraces, three commercial cultivars, and two introduced cultivars from ICARDA were further analyzed to detect variations among durum wheat varieties at DNA level using RAPD technique. The following characters were studied: Plant height, Flag leaf length, and Tillering capacity. Statistical analysis using One Way Anova test showed significant variability among varieties for Tillering number, which ranged between l to 3 tiller/plant. However there was non- significant variability among varieties in plant height and flag leaf length. DNA genetic variation was studied using 11 wheat varieties collected from 7 locations in Palestine. A genetic similarity matrix based on the dice coefficients was constructed using RAPD data to assess the genetic relatedness. The mean similarity indices presented by the 11 varieties ranged from 0.l to 0.81 for all varieties with an average of 0.40. The wide range of similarity indices indicated that a wide range DNA polymorphism occur among the 11 varieties. The results showed also that clustering in the same cluster for the varieties which could have common genomes, but no clustering related to collection site. Of the 11 varieties used, there were 159 RAPD marker produced by five primers. This analysis has proven that RAPD-PCR is a useful tool to determine genetic diversity among durum wheat varieties and can provide valuable information for the management and identification of genetic resources.