Excretory/secretory (E/S) products of Echinococcus granulosus were prepared from presentation stages (PS1, PS2, PS3 and PS4) and one segmented (S5) stage which corresponded to 1-, 5-, 12-, 26- and 43-day old in vitro cultured parasites, respectively. Cultures were initiated from protoscolices collected from hydatid cysts of indigenous Jordanian ewes. The E/S products were characterized using SDS-PAGE with silver, Comassie-blue and PAS staining as well as immunobltting with sera from hydatid patients and immunized mice. Younger cultured stages yielded small quantities of E/S materials, compared to the banding (PS4) and segmented (S5) stages. The E/S products from various stages of cultured parasites were heterogeneous and contained 15-28 protein bands and 3-6-PAS+ bands. A total of 13 protein bands were shared among the E/S products of various cultured stages. 11 of which were also shared with crude sheep hydatid fluid (CSHF), tegumental and somatic antigens. The E/S products of PS1-S5 stages and CSHF shared 3 PAS+ bands. All the immunoreactive components of E/S products except for a band of <14.4 KDa, were detected by sera of unicellular hydatid Jordanian patients and were also reactive to sera of patients infected with E. multilocularis.

Title

Jpn J. Parasitol., (1994) vol. 43. No. 2, 82-89

Publisher

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Publisher Country

Palestine

Publication Type

Both (Printed and Online)

Volume

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Year

2009

Pages

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