Cypress oilwas analyzed using GC-MS,revealingα-pinene (50.72%) and 3-β-carene (27.57%) asthe major constituents.An optimized nanoemulsion (dropletsize 105.28±2.12 nm, PDI 0.112±0.016) wasincorporated into a carbopol hydrogelto form a stable nanoemulgelwith a zeta potential of –33 mV. The nanoemulgel exhibited enhanced antioxidant activity,with an IC50 of 6.6±0.13 μg ml−1 , compared to 14.7±0.3 μg ml−1 forthe oil alone.It demonstrated potent antimicrobial activity against multiple bacterialstrains,with inhibition zones of 11–36 mm, and antifungal activity againstCandida albicans (16–24 mm). Strong anticancer effectswere observed againstHep-G2 (IC 50 39.81 μg ml−1 ),MCF-7 (IC 50 58.88 μg ml−1 ), andHeLa (IC 50 61.65 μg ml−1 ) cells, alongwith anti-fibrotic activity on LX-2 cells(IC 50 63.09 μg ml−1 ). SelectiveCOX-2 inhibitionwas also confirmed (IC50 13.96 μg ml−1 ).These findings highlightthat cypress oil-loaded nanoemulgel effectively enhancesthe biological activities of cypress oil,suggesting its potential as a therapeutic platform for managing infections, inflammation, and cancer

Title

Nano Express

Publisher

IOP Publishing

Publisher Country

United Kingdom

Indexing

Thomson Reuters

Impact Factor

2.5

Publication Type

Both (Printed and Online)

Volume

6

Year

2025

Pages

045012