The current study explores the effectiveness of e-learning in Palestinian and Jordanian universities from the viewpoint of faculty members. To achieve the objectives of the study, the descriptive survey research approach is used. The study sample consisting of 93 faculty members in Palestine and Jordan was selected by the available sampling method. The study instrument represented by a 40-item questionnaire was distributed to the study sample. The findings show the effectiveness of e-learning in Palestinian and Jordanian universities from the viewpoint of the faculty members with a mean of 3.84, which is considered high according to the scale prepared for the study. The findings also indicate that there are no statistically significant differences among the means of the responses of the study sample members regarding the effectiveness of e-learning in Palestinian and Jordanian universities due to the variables (country, university type, and academic rank), where the value of its significance level is greater than (0.05). Considering the previous results, the study recommends working on developing plans and programs for the mechanism of implementing e-learning in Palestinian and Jordanian universities and giving training courses in the domain of e-learning to students and faculty members continuously.