Trichomonas tenax and Entamoeba gingivalis prevalence among Palestinian adults, a PCR-based cross-sectional study
Publication Type
Original research
Authors

Introduction: Periodontal diseases affect the tissues responsible for tooth support within the oral cavity. These conditions are directly connected to tooth loss and other general health issues like cardiovascular diseases. Microorganisms associated with periodontal diseases have been extensively studied; however, parasites like T. tenax and E. gingivalis have been the least investigated and least frequently linked to periodontitis. This research aims to study the prevalence of these parasites in the Palestinian adult community.

Materials and methods: A cross-sectional study was designed to reveal the prevalence of both parasites. Cities to be included in the study were selected via cluster sampling. 291 saliva and plaque scraping samples were extracted from subjects who were eligible and accepted to enroll. Deoxyribonucleic acid (DNA) extraction was done, followed by Polymerase chain reaction (PCR) and agarose gel electrophoresis to detect parasite infection.

Results: After DNA extraction and PCR, all samples were negative for T. tenax and E. gingivalis infection.

Conclusion: The results of this study were insufficient to establish the prevalence of the two parasites among Palestinian adults. Further studies on these parasites and other potential causative agents are recommended.

Journal
Title
European Journal of Dental and Oral Health
Publisher
European Open Science
Publisher Country
United Kingdom
Publication Type
Online only
Volume
6
Year
2025
Pages
35-39