Background The objective of this research is to extract and formulate a nanoemulsion consisting of Vitis vinifera (V. vinifera)
oil. Following this, the antioxidant, antidiabetic, and anti-lipase properties of both the oil and the nanoemulsion system
will be examined.
Method V. vinfera seed oil extraction was followed by the preparation of its self-nanoemulsifying system. GC analysis was
conducted to analyze phytochemical compounds, and physical characterization was performed for the V. vinfera oil selfnanoemulsifying
system. Subsequently, antioxidant, anti-diabetic, and anti-lipase activities were investigated for the oil and
its nanoemulsion.
Result The biologically active components of V. vinifera oil were detected using a GC/MS device. The main components
were: linoleic acid methyl ester, oleic acid methyl ester, palmitic acid methyl ester, stearic acid methyl ester, and elaidic acid
methyl ester. The optimum nanoemulsion formulation, which comprised Tween 80, Span 80, and V. vinifera seed oil had a
PDI of 0.227 ± 0.07 and a droplet size of 192.71 ± 1.8 nm. Porcine lipase inhibition of V. vinifera oil and its nanoemulsion was
detected; the oil showed potent lipase inhibitory activity when compared to orlistat. The self-nanoemulsion has less inhibitory
activity than the oil, with an IC50 equivalent to 13.8 ± 1.5 μg/ml. Also, V. vinifera oil inhibited a-amylase with a weak
IC50 value of 257.03 ± 2.4 μg/ml. The free radical scavenging activity of the extracted V. vinifera oil was estimated using
the DPPH radical method and trolox. The self-nanoemulsion has less inhibitory activity than the oil, with an IC50 value of
13.80 ± 1.5 μg/ml.
Conclusion This study demonstrated the importance of black grape (V. vinfera) oil for treating and preventing obesity, diabetes,
and oxidative stress, as well as how nanoemulsions improve these activities. Therefore, this natural oil is a promising
product for the pharmaceutical industry.

Title

Chemistry Africa

Publisher

Springer Nature

Publisher Country

Germany

Indexing

Thomson Reuters

Impact Factor

2.3

Publication Type

Both (Printed and Online)

Volume

--

Year

2025

Pages

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