Microbore Liquid Chromatography with UV Detection to Study the In Vivo Passage of Compound 21, a Non-Peptidergic AT2 Receptor Agonist, To the Striatum in Rats
Publication Type
Original research
  • Naser Shraim
  • Birgit Mertens
  • Ralph Clinckers
  • Sophie Sarre
  • Yvette Michotte
  • Ann Van Eeckhaut

A microbore liquid chromatography method coupled to UV detection was developed and validated in order to monitor the passage of compound 21 (C21), a non-peptide angiotensin II type 2 receptor agonist, to the striatum of rats. For this purpose, sampling from the striatum was performed using the in vivo microdialysis technique. Separations were performed on a C18 microbore (1 mm i.d.) column using gradient elution. The retention time for C21 was found to be 6.3 min. The calibration curve was linear between 10 and 200 ng/ml with a correlation coefficient ≥0.999. The limit of detection and the limit of quantification were 3 and 10 ng/ml respectively. The intra-day and the inter-day precision (RSD%) ranged between 0.5 and 4.6% with an average recovery of 101.5 ± 10.0% (mean ± SD, n = 15). In vivo experiments were performed on rats to measure the concentration of C21 in striatal dialysates after intraperitoneal (10 or 50 mg/kg) or intravenous injection (10 mg/kg or 20 mg/kg) of C21 and suggest minimal passage of the compound to the striatum.

Journal of Neuroscience Methods Volume 202, Issue 2, 15 November 2011, Pages 137–142
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