Antibacterial activity of peptides from Rosmarinus officinalis leaves in combination with sulfamethoxazole and trimethoprim antibiotics
Publication Type
Original research

Plants are a major source of antimicrobial peptides. So, the aim of this study was to determine the antibacterial activity of peptides isolated from Rosemary (Rosmarinus officinalis) leaves. Such as activity was compared with the synergistic effect of these peptides in combination with sulfamethoxazole and trimethoprim antibiotics against Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, and Staphylococcus aureus. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for each combination were measured using the micro-broth dilution method. For further antibacterial investigation, the fractional inhibitory concentration (FIC) was also used. The obtained results demonstrated the antibacterial potential of rosemary peptides against all tested bacteria. Moreover, results showed a strong inhibitory effect against Gram positive bacterium S. aureus with MIC value of 1.563 mg/mL. Also, MBC results provided that S. aureus was the most sensitive one to rosemary peptides with MBC value of 12.5 mg/mL. The interpretations of the activity of rosemary extract combined with both sulfamethoxzole and trimethoprim produced a remarkable synergistic activity against S, aureus, E. coli and P. aeruginosa. In conclusion, the present research clearly showed that rosemary peptides considered as promising antibiotic compounds that play an important role in the enhancing of susceptibility of some antibiotics

Moroccan Journal of Biology
Moroccan Society of Biology
Publisher Country
Publication Type
Both (Printed and Online)