In vitro Screening of Glutathione- S -Transferase Inhibition by Different Extracts of Verbascum sinuatum
Publication Type
Original research


Background and Aim: The concern toward using herbs as enzyme inhibitors such as glutathione-s-transferases (GSTs) may improve the treatment of proliferative diseases and overcome the problem of multidrug resistance tumors. Based on that, this experiment was carried out to detect the impact of using aqueous, ethanol and methanol extracts from Verbascum sinuatum L. (Scrophulariaceae) that is growing wild in Palestine as enzymatic inhibitors.

Materials and Methods: The impact of the three extracts at four studied concentrations (250, 500, 750 and 1000 µg/mL) on the activity of purified hepatic glutathione-s-transferases was estimated spectrophotometrically by using-chloro-2,4-dinitro-benzene (CDNB) as substrate.

Results: Research results revealed that all three prepared extracts of V. sinuatum inhibited the activity of GSTs at all examined concentrations. Moreover, results provided that the alcoholic extracts from the studied plant species influenced the activity of GSTs more than the aqueous one. However, results indicated that both methanol and ethanol extracts demonstrated minor inhibitory variations between them. It is notable that all studied extracts manifested inhibitory effect in a dose dependent manner.

Conclusion: Results emphasized and underlined the possibility of utilizing V. sinuatum extracts in pharmaceutical industry of new medications to fight drug resistant tumors in general and  GST-induced tumors in particular.

Herbal Medicines Journal
Lorestan University of Medical Sciences
Publisher Country
Publication Type
Both (Printed and Online)