This study aims to identify the barriers and facilitator of using Moodle or e-learning by faculty of Medicine and health Sciences College and Education College. This study contributes to the understanding of faculty perceptions of barriers, the different types of barriers and facilitators that help them to use Moodle or e-learning smoothly. This will help the academic administrator to guild their agenda and aims toward overcoming these barriers and improving the facilitator factors which will help to improve the quality of learning. The most important factor to alleviate poverty and economic growth in developing countries is investing in education. An- Najah National University considered as a big education service provider in Palestine. Improving quality of education services is an important matter.