Damask rose (Rosa x damascena Mill) is an important ancient scented hybrid rose. A direct organogenesis protocol was developed for in vitro propagation using dormant axillary buds. We tested the effect of Murashige and Skoog (MS) and Woody Plant Medium (WPM) supplemented with benzyladenine (BA), kinetin, and thidiazuron at 0.2, 0.5, and 1 mg/L. Shoot induction occurred in 40% of sterilized explants. WPM produced longer shoots and more leaves than MS. Optimal shoot multiplication occurred on WPM supplemented with 1 mg/L BA, 3% sucrose, and 0.7% phytagar. In vitro rooting (50%) was obtained when shoots were transferred to WPM with half-strength macronutrients containing 2% sucrose and 1 mg/L indole-3-butyric acid. Rooted plantlets were transferred to soilless media. The use of a growth chamber with controlled humidity during the first days of acclimatization increased plant survival (93%). Plantlets were subsequently grown to maturity in the glasshouse.

Title

The 4 th International Conference on “Plant Cell & Tissue Culture In Vitro IV”.

Publisher

Vienna International Science Conferences and Events Association (VISCEA), Vienna, Austria.

Publisher Country

Austria

Publication Type

Prtinted only

Volume

--

Year

2022

Pages

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