Introduction. Mollicutes urogenital tract infections are considered a possible cause of infertility worldwide. Genital Mollicutes
infections are difficult and impractical to diagnose by culturing or serology. Mollicutes included in this study were Mycoplasma
hominis, Ureaplasma urealyticum, and Mycoplasma genitalium. *is cross-sectional study aimed to determine the prevalence of
M. hominis, U. urealyticum, and M. genitalium genital infections among infertile males and females patients. Methods. *is study
included 103 patients who visited Al-Shunar Clinic in Nablus city in Palestine and diagnosed with infertility during January 2018
to October 2018. *e semen, urine, and/or vaginal swab specimens collected from patients were examined by PCR for detection of
M. hominis, U. urealyticum, and M. genitalium. Results. A total of 57 semen, 37 urine, and 16 vaginal swab specimens were
collected. Out of the 110 examined specimens, 35 (31.8%) were PCR positive for at least one Mollicutes, which were 16 (14.6%)
M. hominis, 11 (10%) U. urealyticum, and 8 (7.3%) M. genitalium. Significant association were found between infections of
M. hominis and U. urealyticum (P � 0.044) and between M. hominis and M. genitalium (P � 0.005) infections. M. hominis
infection was found in significantly (P � 0.048) higher percentage in males (20.6%) in comparison with females (5.7%). On the
other hand, M. genitalium infection rate in females (8.6%) was slightly higher than males (7.4%). M. hominis was more prevalent in
all age groups except for patient’s age group 40–50 years old, where M. genitalium was more prevalent. M. hominis was also more
prevalent in all occupation types and among all smokers. Conclusion. Urogenital infections caused by M. hominis, M. genitalium,
and U. urealyticum could be a possible cause of infertility among patients with different age groups, genders, and occupations.
*us, more attention by infertility centers and physicians is required in adopting molecular methods for diagnosis of infections by
these microorganisms.

Title

Canadian Journal of Infectious Diseases and Medical Microbiology

Publisher

Hindawi

Publisher Country

United Kingdom

Indexing

Thomson Reuters

Impact Factor

24.0

Publication Type

Both (Printed and Online)

Volume

2022

Year

2022

Pages

7