Background: Microbial resistance, diabetes mellitus, and obesity are global health care problems that have posed
a serious threat to both human and environmental ecosystems. The goals of the present investigations are to investigate
the phytoconstituents, antilipase, anti-α-amylase, and antimicrobial activity of Orobanche aegyptiaca Pers. (OA)
from Palestine.
Methods: Identification of the phytoconstituents of OA plant petroleum ether, methylene chloride, chloroform,
acetone, and methanol extracts were conducted using pharmacopeia’s methods, while porcine pancreatic lipase
and α–amylase inhibitory activities were examined using p-nitrophenyl butyrate and 3,5-dinitro salicylic acid methods,
respectively. Moreover, the antimicrobial activity was evaluated utilizing broth microdilution assay against eight
bacterial and fungal strains.
Results: The phytochemical screening results showed that the methanol extract of the OA plant is rich in phytochemical
components, also this extract has powerful antilipase potential with an IC50
value of 19.49 ± 0.16 μg/ml
comparing with the positive control (Orlistat) which has antilipase activity with IC50
value of 12.3 ± 0.35 μg/ml. Moreover,
the methanol and chloroform extracts have powerful α-amylase inhibitory activity with IC50
values of 28.18 ± 0.22
and 28.18 ± 1.22 μg/ml, respectively comparing with Acarbose which has α-amylase inhibitory activity with IC50
dose
of 26.3.18 ± 0.28 μg/ml. The antibacterial results showed that the methylene chloride extract exhibited the highest
antibacterial activity among the other OA plant extracts with a MIC value of 0.78 mg/ml against S. aureus, while, the
methylene chloride, petroleum ether, and chloroform extracts of the OA plant showed potential antifungal activity
against C. albicans strains with MIC value of 0.78 mg/ml.
Conclusion: The OA methanol and chloroform extracts could be excellent candidates as antilipase and anti-α-
amylase bioactive materials. In addition, methylene chloride, petroleum ether, and chloroform extracts could be
potential natural antimicrobial products.

Title

BMC Complementary Medicine and Therapies

Publisher

Biomed Central

Publisher Country

United Kingdom

Indexing

Thomson Reuters

Impact Factor

3.4

Publication Type

Both (Printed and Online)

Volume

21

Year

2021

Pages

1-8