The aim of the present study was to investigate some biological activities of Aizoon hispanicum, Heliotropim maris-mourtui and Polygonum arenarium which are wildly growing in Palestine. The first part of the current research was to find out the effect of the screened plant extracts on hepatic GSTs spectrophotometrically. The obtained results revealed that all examined different aqueous extracts from the plant species under study demonstrated inhibitory effect on GSTs activity at all tested concentrations. Among all the investigated plant extracts, result showed that A. hispanicum exhibited the strongest inhibitory effect on GSTs activity. The second part of this study was to confirm the antioxidant activity of the investigated plant species through DPPH assay. P. arenarium aqueous extract showed the highest antioxidant activity which is relatively similar to the antioxidant power of ascorbic acid. The last part of this study was conducted to evaluate the antibacterial effect of the three extracts against six different bacterial isolates using broth dilution method. The obtained antibacterial results indicated that all examined extracts were able to act against all studied bacteria with observed variation among them. In conclusion, results indicated that the extracts of the tested plant species could be explored as natural remedies for health promotion.