Dogs play an important role in transmission of parasites and zoonotic diseases, especially in developing countries. The objective of this study was to investigate the prevalence of internal parasites in dogs in Palestine. The targeted dogs were both domestic and stray dogs according to gender and age. Fecal samples were collected during the period between January to May 2019. A total of 150 fecal samples were collected and tested for parasites using sedimentation and flotation techniques. Although there was no significant difference in infestation between males and females, the prevalence rate of infestation in males was 63.1% compared to females 72.4%. Dogs of ages under one year had similar rate of infestation compared to older dogs with a rate of 67.3% and 67.4% respectively. Infestations were significantly higher (P<0.05) in stray dogs 48.4%, compared to domestic dogs 81.4%. The highest infestation rate was with Toxocara canis 46.0% followed by Dipylidium caninum 23.0%, Echinococcus sp. 14.0% ,Isosopora canis 9.0%, Ancylostoma caninum 8.0%, Giarda sp. 5.0%, Strongyloides sp. 4.0%, Trichuris vulpis 3.0%, Cryptosporidium sp. 3.0%. Results of this study are the first to shed light on intestinal parasites infestation in native dogs. Future plans should be proposed and applied by authorities to achieve a healthy status of residence in the country.