In Vitro Inhibition of Sheep Liver Glutathione-S-Transferases Activity by Different Extract Types from Ephedra aphylla and Ephedra foeminea
Publication Type
Conference Paper

Glutathione-S-transferases (GSTs) play an important role in the detoxification of chemicals that may lead to mutagenic or cytotoxic effect. In this study, we aimed to find the effect of aqueous, methanol and ethanol extracts of both Ephedra aphylla and Ephedra foeminea on purified sheep liver GST activity at the following different concentrations (100, 200, 400, 600, 800 and 1000 µg/ml) spectrophotometrically using CDNB as substrate. The obtained results revealed that all examined different extract types of Ephedra species under study manifest inhibitory effect on GSTs activity at all screened concentrations. The inhibitory effect on GST activity by the alcoholic extracts of the two species under investigation at all considered concentrations is more than the aqueous ones in comparison to the controls. Even it was observed that the lowest concentration (100 µg/ml) of both the methanol and ethanol extracts of both plant species exhibited higher inhibitory effect than the highest concentration (1000 µg/ml) of the aqueous ones. This is due to that the methanol and ethanol extracts of E. aphylla and E. foeminea showed 43, 31, 40 and 24 inhibition % at 100 µg/ml, respectively. While, the aqueous extracts of them showed 25 and 21 inhibition % at 1000 µg/ml, respectively. Nevertheless, this is more pronounced in E. aphylla rather than in E. foeminea. On the other hand, both methanol and ethanol extracts showed minor variations between them in both E. aphylla and E. foeminea. Contrary to that, the aqueous extract of E. aphylla was of higher recognized inhibitory effect than E. foeminea compared to the control. It is worth mentioning that all the GSTs inhibitory effects displayed dose dependent manner under all studied extracts. On that account, the obtained results in this research confirms the medicinal importance of both Ephedra species under study, which provides the possibility of their use in whatever benefit human health in general and combat GST-induced resistance in drug resistant tumors in particular. On the other hand, their consumption should be under professional’s supervision as their GSTs inhibition effect would in turn reduce the therapeutic effects of drugs or even cause therapeutic failure as the residence time of the drug in the body is reduced. Last but not least, in vivo studies concerning the effect of the examined plant species different extracts on the activity of GSTs are sought after in addition to their mechanism of action. 

Conference Title
The 4th international medical science conference of An-Najah national university
Conference Country
Conference Date
Oct. 27, 2019 - Oct. 27, 2019
Conference Sponsor
جامعة النجاح الوطنية \كلية الطب