The effect of Brassica oleracea (Cabbage) on the pharmacokinetics of ciprofloxacin was studied in rabbits. A single dose of Ciprofloxacin (40mg/kg) was given to rabbits as a suspension along with an aqueous extract of Brassica oleracea (var. capitata) equivalent to 2g/kg. Twelve rabbits were sampled in a two-period, two-sequence, crossover study, with 7day washout period. Blood samples were obtained at (t = 0, 5, 15, 25, 40, 60, 90, 120, 180, 240, 360, 480 and 580 minutes), and analyzed using a validated HPLC method. A reversed phase high performance liquid chromatographic method (RP-18) was used for the determination and quantification of plasma ciprofloxacin concentrations. The minimum quantifiable concentration of ciprofloxacin was 6 ng/ml (LOQ), the limit of detection (LOD) was 2 ng/ml, and calibration curves were linear over the range 0.01-8.0 µg/ml, with correlation coefficients >0.999. Both, the between-day coefficient of variation (interday RSD) and within-day coefficient of variation (intraday RSD) for quality-control samples were less than 5%. The pharmacokinetic values for ciprofloxacin were estimated by noncompartmental methods. When co-administered with the cabbage extract, Tmax for ciprofloxacin in plasma was reduced to the half; Cmax was increased by 1.5 times and AUC was 1.32 times higher. Results obtained indicated a potentiating effect of Brassica oleracea (Cabbage) on the ciprofloxacin which could be a result of the effect of glucosinolate and brassicasterol found in cabbage.

Title

Jordan Journal of Pharmaceutical Sciences

Publisher

University of Jordan

Publisher Country

Jordan

Indexing

Scopus

Impact Factor

None

Publication Type

Both (Printed and Online)

Volume

12

Year

2019

Pages

59-67