Leguminosae or Fabaceae is the third largest flowering plant family. It is economically important for food production and soil fertility. The molecular phylogenetic analysis of closely related species of Leguminosae family: Lens culinaris, Vicia sativa, V. palaestina, V. peregrina, V. faba, V. narbonensis, Lathyrus aphaca, Pisum fulvum and P. sativum aids in the discrimination among these closely related species. In this study, 18S and 28S as universal primers were used for amplifying and sequencing the internal transcribed spacer (ITS) region of the studied species. This was conducted on around two-five plant individual samples of each one of the species under investigation. The phylogenetic tree construction was carried out using Unweighted Pair Group method. The phylogenetic analysis among the studied species revealed that V. peregrina, V. faba, V. narbonensis, V. palaestina, Lens culinaris, and V. sativa were grouped into one clade (clade І). However, V. sativa occupied the farthest position in clade І, and was separated from other species of Vicia genus. P. sativum, P. fulvum and Lathyrus aphaca were grouped into another clade (clade ІІ). On the other hand, Lens culinaris occupied a position within the core of Vicia near to V. palaestina. Accordingly, it is recommended to transfer Lens culinaris into Vicia genus based on its morphological and molecular characterization. Therefore, the new classification of Lens culinaris is recommended to be Vicia culinaris.