This course is designed to provide students with the basic skills needed to manipulate mammalian cells in culture. Laboratory skills in cell growth, cell passage, gene transfection, and enzyme assays will be acquired. Since this is a upper level course, the student should be familiar with common biological terms.
All the material covered in the exams will be based on information from the textbook and laboratory exercises. In order to do well in this course, the assigned reading must be read before lecture to help you grasp the problems and to ask for clarification of concepts when necessary.
The goal of Animal Cell Culture Techniques is for students to acquire the necessary practical skills for the isolation of mammalian cells for in vitro studies, maintenance of animal cells in vitro, manipulation of animal cells in vitro, and application of molecular techniques to in vitro situations. Lectures provide the background to understand the basic principle of cell culture.
Upon completion of this course, students should be able to:
Culture of Animal Cells: A Manual of Basic Technique (6th Edition) R. Ian Freshney. REQUIRED. It is in your best interest to bring this book or the required chapters to class.
Notebook REQUIRED. All lab procedures and recording of data will be in this lab notebook.
Laboratory Safety: Students are expected to behave professionally at all times and follow laboratory safety rules.
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