Course Title

Laboratory of Applied Biotechnology 2

Course Number

24487

Instructor Name

Motasem Al-Masri

Contact Information

[email protected]

Semester(s) and academic year(s)

First Semester 2016
Second Semester 2015
First Semester 2015
Second Semester 2014
First Semester 2014
Second Semester 2013
First Semester 2013

Compulsory / Elective

Compulsory

Laboratory of this course include several experiment, which includes antigen, animal bleeding and immunization, agglutination test, double diffusion, double diffusion staining, immunoelectrophoresis, autoimmune disorders- Rheumatoid factor, vaccine preparation, rocket electrophoresis, cellulose acetate electrophoresis, ELISA and IFA.

At end of the course the student must have basic knowledge of:

1. Animal bleeding and immunization.
2. Detection of antigen-antibodies reactions by agglutination test, double diffusion and immunoelectrophoresis.
3. Diagnosis of autoimmune disorders such as Rheumatoid factor.
4. Vaccine types and preparation.
5. Detection of certain proteins.
6. Detection of specific subtypes of antibodies by ELISA and IFA.

Textbooks:

Immunology. David Male, Jonathan Brostoff, David B Roth, Ivan M Roitt. 8^th Ed.   2013.

Basic Immunology Abul K. Abbas  and Andrew H. Lichtman.  3^thEd.  2011