One of the latest tactics in cancer therapy is the application of siRNA to regulate gene
expression. Nonetheless, the efficient administration of siRNA into cells presents a
significant problem, especially in vivo. Researchers are creating innovative technology
delivery ways to transport these chemicals with minimal impact. The advancement of drug
delivery methods utilizing carbon nanotubes has garnered significant attention in this
context. Our project seeks to establish a novel technique for the delivery of siRNA into
colon cancer cells to specifically target the expression of the β-catenin protein, which is
implicated in numerous developmental processes, including cell growth and proliferation,
embryonic patterning, cell differentiation, and various cellular functions. The novel
methodology relies on the creation of a new nano-system comprising multi-walled carbon
nanotubes (MWCNTs) functionalized with a tetra-amine linker and mannose sugar as a
targeting agent to enhance the nano-system's uptake and selectivity. We effectively
functionalized MWCNTs with tetra-amine groups, subsequently attaching mannose
molecules. The quantities of mannose and amine-conjugated groups were also measured.
The N/P ratio necessary for the entrapment of β-catenin siRNA was ascertained using
agarose gel electrophoresis. Western blot investigation demonstrated that our product
markedly decreased β-catenin protein expression (p > 0.05). The knockdown rate was 50%
for f-MWCNTs. We predicted that the potential upregulation of mannose receptors on the
surface of Caco-2 cells may enhance the transfection effectiveness of f-MWCNTs. The
MTS results indicated that the down-regulation of β-catenin protein can inhibit cancer cell
growth and limit cell proliferation, as evidenced considerably in both transfection methods,
with an IC50 of 19.32 μg/ml for f-MWCNT-siRNA. This action can greatly enhance the
efficacy of 5-FU in colon cancer compared to the control group. Consequently, we suggest
that our methodology may be applied in the context of colon cancer treatment.

عنوان المؤتمر

Zipc 2024

دولة المؤتمر

فلسطين

تاريخ المؤتمر

1 يناير، 2028 - 1 يناير، 2028

راعي المؤتمر

Al-Zaytoonah University