Introduction: In Palestine, Verbascum fruticulosum is one of the most frequently used traditional medicinal plants.
The objective of this study was to analyze the contents of the V. fruticulosum extract and evaluate its biological
properties.
Methods: Plant total phenol, flavonoid, and tannin contents were estimated using Folin–Ciocalteu’s, aluminum
chloride, and vanillin-hydrochloride colorimetric assays, respectively. Reference biomedical assays were per-
formed to evaluate the cytotoxic, antimicrobial, DPPH free radical scavenging, antilipase, α-amylase, and
α-glucosidase inhibitory effects of the extracts.
Results: The methanol and acetone extracts exhibited the highest antibacterial activity against Pseudomonas
aeruginosa with a Minimum Inhibitory Concentration (MIC) value of 1.56 mg/mL for both extracts. The n-hexane
extract demonstrated the most potent activity against Epidermophyton floccosum fungi, with a MIC of 3.13 mg/
mL. The aqueous extract exhibited excellent antioxidant activity with a Half-maximal Inhibitory Concentration
(IC50) of 0.71 ± 0.31 μg/mL. The acetone extract displayed the highest inhibitory activities against lipase and
α-amylase, with IC50 values of 129.05 ± 0.48 μg/mL and 9.01 ± 1.76 μg/mL, respectively. The plant methanolic
extract presented the highest inhibitory effect against α-glucosidase (IC50 = 75.46 ± 0.66 μg/mL) and the
acetone extract exhibited the highest cytotoxic effect (IC50 =3.79 ± 0.01 mg/mL).
Conclusion: This study is the first to investigate the phytochemistry and inhibitory activity of V. fruticulosum
against lipase, α-amylase, and α-glucosidase. The results indicate that V. fruticulosum extracts exhibit potential
biological activities. Further, in vivo trials are necessary to validate these bioactivities.
 

Title

European Journal of Integrative Medicine

Publisher

Elsivere

Publisher Country

Germany

Indexing

Thomson Reuters

Impact Factor

2.5

Publication Type

Both (Printed and Online)

Volume

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Year

--

Pages

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