Potato production is highly affected by viral diseases such as potato virus X (PVX) and potato virus Y (PVY) which can be overcome by establishing a  reproducible meristem culture protocol for the production of virus-free potato plants. This study was conducted during 2017 and 2018 on two potato cultivars “Spunta” and “Alaska Red” in Palestine. The tubers were subjected to reverse transcriptase-polymerase chain reaction (RT- PCR) for detection of virus  infection. The infected tubers for both cultivars were incubated, and after sprouting, meristems-tips were isolated and cultured on Murashige and Skoog (MS) medium. After four weeks, developed shoots were cultured on MS media
supplemented with different rates of three hormonal combinations (0.54 µM NAA; 0.54 µM NAA + 1.0 µM TDZ; 0.54 µM NAA + 2.2 µM BA; 0.54 µM NAA + 2.3 µM Kinetin; 0.54 µM NAA + 1.0 µM TDZ+ 2.2 µM BA + 2.3 µM Kinetin). The results showed that maximum shoot multiplication was observed when NAA at 0.54 µM was combined with 1 µM TDZ (2.85 and 2.55 shoots) in both “Spunta” and “Alaska Red” cultivars, respectively. Higher shoot length and leaf number were obtained when NAA was combined with either BA (2.2 µM) or Kinetin (2.3 µM) in both cultivars. In vitro multiplied shoots were rooted and acclimatized successfully. Neither t PVY nor PVX viruses were detected by RT-PCR on the RNA extracts from the tested in vitro grown samples 

Title

Research on Crops

Publisher

Gaurav Publications.

Publisher Country

India

Indexing

Scopus

Impact Factor

None

Publication Type

Both (Printed and Online)

Volume

23

Year

2022

Pages

363-369