Background: Nigella sativa L. (N. sativa) has been reported to have biological activities such as anti-bacterial, anti-inflammatory, antioxidant
and anti-fungal activities. Objective: This study aims to develop N. sativa colloidal-emulgel with evaluation of its anti-bacterial,
anti-oxidant and in-vivo irritation and sensation testing. Method: Colloidal-emulgel formulations were prepared for N. sativa using
different surfactants (Sodium Lauryl Sulphate (S.L.S) and sucrose ester). N. sativa emulsion formulations were prepared using heat
inversion technique. After that the optimum formulation was mixed with Carbopol to produce the colloidal-emulgel. The droplet size,
size distribution and rheological behavior were measured for emulgel formulations. Anti-bacterial and anti-oxidant activities were
conducted also the in vivo studies for sensitivity, irritancy and spreadability were reported. Results: It is found that sucrose ester was
able to produce the optimum emulsion formulation with droplets size of less than 1 μm. In anti-bacterial test for Staphylococcus
aureus, it was found that emulgel has inhibition zone of 2.5 cm in diameter, but the oil alone 1.3 cm. According to MRSA the inhibition
zone for emulgel was 1.1 cm, but for oil 0.5 cm in diameter. Emulgel do not show any irritations or sensitivity also it has homogeneous
appearance with smooth texture. In addition, it shows fair mechanical properties, easily spreadability with acceptable bio-adhesion.
Conclusion: It is concluded that N. sativa emulgel has been prepared with dermatological and cosmeceutical benefits.