Introduction

Bioactive ingredients of camel milk and plant extracts have gained significant attention of scientists for their medicinal values. Therefore, the present study was performed to evaluate the antibacterial potential of camel whey and Rosmarinus officinalis extracts. In addition to their effect on the activity of Glutathione-S- transferases (GSTs) enzymes.

 

Methods

Whey proteins were prepared by casein precipitation from skimmed milk sample using rennin.  Protein contents of whey were fractionated by SDS-PAGE. Aqueous and ethanol extracts from R. officinalis were prepared to a final concentration 200 mg/ml and 1 mg/ml. The antibacterial activity of all samples and combinations was tested against Staphylococcus aureus and Escherichia coli using disk diffusion method. For further antibacterial investigation, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured by micro-broth dilution method. Furthermore,  GSTs enzymes were extracted and purified from sheep liver using ammonium sulfate precipitation, gel filtration and affinity column chromatography. The effect of camel whey and plant extracts on GSTs was studied by spectrophotometric method using 1-chloro-2,4-dinitro -benzene (CDNB) as substrate.

 

Results

The obtained results showed that ethanol extract was the most effective when compared to the aqueous extract and whey proteins. Synergistic antibacterial activity was observed for whey and ethanol extract against E. coli with MBC value equal to 0.39 mg/ml. In the contrary, whey combination to aqueous extract showed antagonistic activity. Moreover, results revealed that all samples with 1 mg/ml concentration increased the activity of GSTs. The recorded GSTs activity was higher after combination of whey to both extracts. The best results obtained after combination of ethanol extract to whey proteins.

 

Conclusions

The obtained results form a platform for further bioactivity studies using different combinations between camel whey and other plant extracts. In addition to that,  active ingredients and their biological combinations effect should be also studied.

 

عنوان المؤتمر

The 10th International Palestinian Conference of Laboratory Medicine – The 15th Arab Conference of Clinical Biology

دولة المؤتمر

فلسطين

تاريخ المؤتمر

18 إبريل، 2018 - 21 إبريل، 2018

راعي المؤتمر

نقابة الطب المخبري الفلسطينية

معلومات إضافية

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