This study aimed to evaluate the relation bet -
ween the phenotypic antibiotic susceptibility pat -
terns and the antibiotic resistance genes and to
investigate the prevalence of macrolide, linco sa -
mides, strepto gramin, aminoglycoside and tetra cy -
cline resistance genes among MRSA isolates. A total
of 55 clinical MRSA isolates were included in this
study, antibiotic resistance was conducted by
Kirby-Bauer disk diffusion method, broth microdilution
assay and multiplex PCR technique. Our results
showed that there was no discordance between
conventional susceptibility testing and gene detection
by multiplex PCR assay.
The prevalence of erm(A), erm(C), tetK, tetM,
aacA-aphD, vat(A), vat(B) and vat(C) gene among
MRSA isolates was 30.9%, 74.5%, 76.4%, 16.4%,
74.5%, 1.8%, 0% and 5.5%, respectively. These
MRSA strains belonged to SCCmec types II, III, IVa
and V. Rapid and reliable method for antibiotic susceptibility
is important to determine the appropriate
therapy decision. Multiplex PCR can be used for
confirmation of the results obtained by disk diffusion
method or could be used as an alternative diagnostic
method in the routine diagnosis for rapid, sensitive,
and specific detection of MRSA associated antibiotic
resistance genes.

العنوان

Romanian Archives of Microbiology and Immunology, 2014; 73(1-2):9-18

الناشر

Institutul Cantacuzino

بلد الناشر

رومانيا

Indexing

Scopus

معامل التأثير

None

نوع المنشور

Both (Printed and Online)

المجلد

73

السنة

2014

الصفحات

9-18