Molecular characterization of three common olive (Olea europaea L.) cultivars in Palestine using simple sequence repeat (SSR) markers
نوع المنشور
بحث أصيل
  • Ramiz Obaida
  • Hassan Abu-Qaoud
  • Rami Arafeh
النص الكامل

Eight accessions of olive trees from three common varieties in Palestine, Nabali Baladi, Nabali Mohassan and Surri, were
genetically evaluated using five simple sequence repeat (SSR) markers. A total of 17 alleles from 5 loci were observed in
which 15 (88.2%) were polymorphic and 2 (11.8%) were monomorphic. An average of 3.4 alleles per locus was found
ranging from 2.0 alleles with the primers GAPU-103 and DCA-9 to 5.0 alleles with U9932 and DCA-16. The smallest
amplicon size observed was 50 bp with the primer DCA-16, whereas the largest one (450 bp) with the primer U9932.
Cluster analysis with the unweighted pair group method with arithmetic average (UPGMA) showed three clusters: a cluster
with four accessions from the ‘Nabali Baladi’ cultivar, another cluster with three accessions that represents the ‘Nabali
Mohassen’ cultivar and finally the ‘Surri’ cultivar. The similarity coefficient for the eight olive tree samples ranged from a
maximum of 100% between two accessions from Nabali Baladi and also in two other samples from Nabali Mohassan, to a
minimum similarity coefficient (0.315) between the Surri and two Nabali Baladi accessions. The results in this
investigation clearly highlight the genetic dissimilarity between the three main olive cultivars that have been misidentified
and mixed up in the past, based on conventional morphological characters

Biotechnology & Biotechnological Equipment
بلد الناشر
نوع المنشور
Both (Printed and Online)