Objective: To evaluate the difference between testosterone and total prostate specific antigen (TPSA) serum levels between Arabic and Turkish people in the Middle East.

Material and methods: The study included 3 groups of healthy men; Group I included 119 patients with a mean age of 52.73±7.53 years from east of Turkey. Group II consisted of 196 patients with a mean age of 50.32±7.84 years from west of Turkey. Group III consisted of 388, with mean age of 51±6 years from west bank in Palestine. The mean values of PSA and testosterone were calculated for each groups and a comparison were carried out. The relationships among testosterone and TPSA levels and patients’ age were investigated.

Results: The mean TPSA levels for Group I, Group II and group III were 1.11±0.78 ng/mL and 1.75±1.06 ng/mL and 1.1+8 ng/ml respectively (p=0.5, p=0.7, p=0.8 respectively). The mean testosterone levels in Group I (386.4±154.6 ng/dL), Group II (383.9±170.6 ng/dL) and group III (380±230 ng/L) showed no significant difference (p=0.89, p=0.29, p=0.7). In Group I there was a positive correlation between age of the patients and testosterone level (r=0.22, p=0.015); however, in Group II, and group III there were significant negative correlation between age and serum testosterone levels (r=-0.16, p=0.022 and r=-0.29, p=0.01). Serum testosterone level showed no significant correlation with TPSA level in all groups.

Conclusion: Testosterone or TPSA levels did not change among the groups. However, the effect of age on testosterone levels varies according to different geographical regions.