The taxonomic position of six Lactobacillus amylophilus strains, isolated from swine waste-corn fermentations was reinvestigated. All strains were included in a multilocus sequence analysis (MLSA) study for species identification of Lactobacillus using the genes coding for the phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA). The pheS and rpoA partial gene sequences showed that strains LMG 11400 and NRRL B-4435 represent a separate lineage that is distantly related to the type strain of L. amylophilus LMG 6900T and to three other strains of the species. The MLSA data showed that the two strains LMG 11400 and NRRL B-4435 constituted a distinct cluster, yielding 100 % pheS and rpoA gene sequence similarities among each other. The other reference strains clustered together with the type strain of L. amylophilus LMG 6900T and clearly differentiated from the strains LMG 11400 and NRRL B-4435 (80 % and 89 % pheS and rpoA gene sequence similarities, respectively). The 16S rRNA gene sequences of the latter two strains are 100 % identical with the nearest phylogenetic neighbour L. amylophilus showing only 97.2 % 16S rRNA gene sequence similarity. Further polyphasic taxonomic study based on whole-cell protein fingerprinting, DNA-DNA hybridization and biochemical features demonstrated that the two Lactobacillus strains represent a single, novel Lactobacillus species, for which the name Lactobacillus amylotrophicus sp. nov. is proposed. The type strain is LMG 11400T (= NRRL B-4436T= DSM 20534T)

العنوان

Int. J. Syst. Evol. Microbiol. 56, 2523–2527

الناشر

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بلد الناشر

فلسطين

نوع المنشور

Both (Printed and Online)

المجلد

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السنة

2006

الصفحات

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