The present study was conducted to assess the susceptibility of seven local wheat cultivars from Palestine to infection with black stem rust caused by Puccinia graminis f. sp. tritici. Two techniques of disease inoculation were applied during bioassays: global inoculation of entire wheat plants with urediospores and localized inoculation with urediospores on wheat leaf-pieces incubated under humid conditions. Susceptibility of tested cultivars was evaluated according to disease scale based on number and size of typical uredial pustules that appeared after inoculation on entire plants or leaf-pieces. Results obtained on bioassay of susceptibility and disease rating on entire plants indicated that Anbar, Kamatat and Hetiya safra cultivars were the least susceptible to P. g. tritici infection, whereas Debiya beda cultivar was the most susceptible. The other tested cultivars such as Nab-El-Jamal, Debiya sawda and Senf 870 were moderately susceptible. On leaf-pieces, Anbar and Kamatat were the least susceptible cultivars, whereas Debiya beda and Nab-El-Jamal were the most susceptible cultivars. The other tested cultivars such as Debiya sawda, Serif 870 and Hetiya safra were moderately susceptible. Significant reductions were obtained for the size of uredial pustules formed on leaf-pieces when inoculated in an unwounded state compared to the wounded, indicating the importance of wounds during inoculation. The global results indicated the possibility of using the above method of disease inoculating, scaling and rating for evaluation of wheat cultivars susceptibility for the eventual use in breeding program for resistant varieties in Palestine.

العنوان

Arabian Gulf Journalfor Scientific Research, 25 (3): 95-102

الناشر

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بلد الناشر

فلسطين

نوع المنشور

Both (Printed and Online)

المجلد

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السنة

2007

الصفحات

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