Tomato, a top cash crop, is infected by a number of viruses that cause drastic yield losses.
Recently an unusual viral syndrome that resembled somewhat that induced by tobacco mosaic
virus has been observed in Northern Palestine. A study aimed at revealing the cause of the
disease, identified the presence of an apparently undescribed tobamovirus. The virus genome
was entirely sequenced and shown to be composed of 6391 nucleotides. Sequence analysis
indicated that this virus was an isolation of tomato brown rugose fruit virus (TBRFV). This is
the first time TBRFV was detected in Palestine on tomatoes and the name tomato brown rugose
fruit virus-Palestinian isolate (TBRFV-Ps) is suggested. Molecular tools were developed for
specific detection of the virus. Moreover, 15 different commercial tomato varieties have been
selected which are commonly cultivated by the Tomato farmers to be assessed for their
tolerance to TBRFV infection using NGS technology. The cultivated plants were inoculated
with TBRFV and the typical viral symptoms had appeared on the different varieties. The plant
samples were collected at different times of the growing period to assess and quantify the viral
infection. The NGS sequence data was transformed into actual viral load for each individually
analyzed sample. The results of the NGS analysis indicated that 8 varieties reflect the case of
tolerant plants. Based on our results, NGS technology is considered the most sensitive and new
comprehensive approach for diagnosis of plant viruses.

العنوان

5th AAB PlantEd Congress: Agricultural Biotechnology in the Era of Genome Editing. 30 October 2024 – 1 November 2024

الناشر

Ondokuz Mayıs University.Atatürk Congress Center.Samsun.Türkiye

بلد الناشر

تركيا

نوع المنشور

مطبوع فقط

المجلد

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