Molecular detection and identification of citrus bent leaf viroid (CBLVd) and hop stunt viroid (HSVd) in Palestine
نوع المنشور
بحث أصيل
المؤلفون
النص الكامل
تحميل

citrus plants are natural hosts to several phytopathogens, including viroids, many of which have a significant economic impact on the crop. this research was to identify molecularly the citrus bent leaf viroid (cBlVd) as well as hop stunt viroid (hsVd) in citrus in Palestine. Field inspections were conducted during august 2020; where virus and virus-like symptoms were noted as epinasty; vein discoloration and leaf deformation were noticed in the citrus germplasm collection in the National agriculture Research center (NaRc). Using a two-step Rt-PcR detection method, hsVd was detected in 9.5% of samples and cBlVd in 7%. the amplified amplicons of both detected viroids were sequenced and deposited in gene bank as Palestinian isolates under the accession numbers PQ084650 and PQ084651, respectively. to our knowledge, this is the first molecular iden tification of cBlVd and hsVd in citrus in Palestine.

المجلة
العنوان
Archives of phytopathology and plant protection
الناشر
Taylor and Francis
بلد الناشر
المملكة المتحدة
نوع المنشور
Both (Printed and Online)
المجلد
58
السنة
2025
الصفحات
356-364