The following experiment investigated the effect of meropenem antibiotic on the activity of glutathione-S-transferase (GST) isoenzymes. This effect was estimated spectrophotometrically at different concentrations of meropenem using 1-chloro-2,4-dinitrobenzene (CDNB) as a substrate. The obtained results revealed that meropenem antibiotic demonstrated an inhibitory effect on GST activity at low tested concentrations (0.25, 0.5, 1 and 2.5 mM) that resulted in 30.5, 39.4, 45.1, and 100% inhibition, respectively. Also, results provided that the meropenem concentration required to reduce the GST activity to half equal to 1.266 mM. After applying the Line weaver-Burk blot and equation, it was noted that both Km and Vmax values decreased in the presence of meropenem, indicating uncompetitive inhibition. In conclusion, meropenem antibiotic is a potential inhibitor for GST. Therefore, it will be crucial to consider the metabolic defense systems when selecting the dosages of this antibiotic to be utilized for the treatment of infections.

العنوان

Palestinian Medical and Pharmaceutical Journal

الناشر

An-Najah National University

بلد الناشر

فلسطين

Indexing

Scopus

معامل التأثير

None

نوع المنشور

Both (Printed and Online)

المجلد

10

السنة

2025

الصفحات

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