Abstract Multiple Sclerosis (MS) is an autoimmune disease characterized by recurrent episodes of central nervous system (CNS) demyelination leading to variable clinical symptoms. The exact etiology and pathogenesis of the disease still remains unknown; however, it is believed to occur as a result of genetic, environmental and immunological factors. Change in the size of brain structure is an important issue in patient with multiple sclerosis. Hippocampal atrophy is the main interested problem in the hippocampus. The aim of the study to find if there’s a relationship between multiple sclerosis and associated changes in the brain structure (hippocampus and temporal lobe) which can use later to find if these changes affect patient memory and learning ability. Magnetic resonance imaging (MRI) was used to obtain brain images for a total of 5 patient (3 females and 2 males) diagnosed with definite MS according to An –Najah National University Hospital with age between (18 - 40). A total of 5 healthy volunteers with no previous history of neurological dysfunction were the control subject (3 males and 2 females) with a range of age between (18-40). A T1 coronal weighted images were acquired in a1.5 Tesla Philips scanner. It was found that there’s a change in the size of the hippocampus in patient with multiple sclerosis compared to the normal patient with the right side of the hippocampus and have higher percentage of volume loss compared to the left side. Based on the above result, multiple sclerosis patient suffers from volume loss in hippocampus