Molecular detection of Citrus exocortis viroid (CEVd), Citrus viroid‑III (CVd‑III), and Citrus viroid‑IV (CVd‑IV) in Palestine
نوع المنشور
بحث أصيل
المؤلفون
النص الكامل
تحميل

Citrus hosts various phytopathogens that have impacted productivity, including viroids. Missing data
on the status of viroids in citrus in Palestine were not reported. This study was aimed to detect any of
Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd‑III), and Citrus viroid-IV (CVd‑IV) in the Palestinian
National Agricultural Research Center (NARC) germplasm collection Field inspections found symptoms
such as leaf epinasty; vein discoloration, and bark cracking on various citrus varieties. RT‑PCR revealed
a significant prevalence of CVd‑IV; CEVd and CVd‑III (47%, 31%, and 22%; respectively). CVd‑III variants
with 91.3% nucleic acid sequence homology have been reported. The sequence of each viroid were
deposited in GenBank as (OP925746 for CEVd, OP902248 and OP902249 for CVd‑III‑PS‑1 and ‑PS‑2
isolates, and OP902247 for CVd‑IV). This was the first to report three of citrus viroids in Palestine,
appealing to apply of phytosanitary measures to disseminate healthy propagating materials free from
viroids.

المجلة
العنوان
Scientific Reports
الناشر
Nature
بلد الناشر
المملكة المتحدة
Indexing
Thomson Reuters
معامل التأثير
4,6
نوع المنشور
Both (Printed and Online)
المجلد
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السنة
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الصفحات
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