Water pipe smoking is highly prevalent in developing countries, especially in Eastern Mediterranean
regions. Research finds that more than 100 million people smoke a water pipe. Furthermore, tobacco
smoking is one of the leading behavioral factors related to an increased risk of cancer, a leading cause
of death globally. We aim to introduce a novel filtration system for water-pipe smoking and evaluate
cytotoxic effects of common water pipe condensed smoke in comparison with our novel filtration system
on normal (HEK293t) and cancer cell lines (Hep3B and MCF7) by MTS assay, alpha-fetoprotein
(aFP), and apoptosis/necrosis effects. More so, the smoke substituents’ neurotoxicity effect was evaluated
by analyzing the depressive property on AMPA receptors (AMPARs). Our results showed that the
silica filtration system was more effective than the water filtration system. The number of toxic compounds
was reduced from 145mg in distilled water extract (DWE) to 57.5mg in silica solution extract
(SSE). The SSE method also showed lower toxicity impacts on normal and cancerous cell lines
(HEK293t, Hep3B, and MCF7) with CC50 values 149.9, 10.14, and 8.9 mg/ml, relative to the DWE method
(CC50 values 77.1, 3.1, and 5.24 mg/ml, respectively). SSE extraction also reduced the a-FP (tumor marker
test) to 2273.3 ng/ml which was closer in value to untreated cells (4066.7 ng/ml) in comparison with
DWE which reduced it greatly to 1658.7 ng/ml, and the biophysical properties of AMPAR subunits demonstrate
a reduced effect on desensitization rates of GluA2 homomer and GluA1/2 heteromer, using
SSE relative to DWE. In conclusion, the condensed smoke of ordinary water pipe (DWE) has cytotoxic
and neurotoxic impacts on various cell lines, while our newly developed system (SSE) was less toxic.

العنوان

DRUG AND CHEMICAL TOXICOLOGY

الناشر

Taylor & Francis Group

بلد الناشر

الولايات المتحدة الأمريكية

Indexing

Thomson Reuters

معامل التأثير

1,9

نوع المنشور

إلكتروني فقط

المجلد

2021

السنة

2021

الصفحات

1-9