Alfa-amylase inhibitors are used to reduce glucose absorption by suppressing carbohydrate digestion. The current
study aimed to evaluate seven wild edible Palestinian plants’ hydrophilic and lipophilic fractions against
porcine pancreatic α-amylase enzyme. The lipophilic fractions of Arum palaestinum, Malva sylvestris, Plantago
major, Centaurea iberica, Cichorium endivia, Bituminaria bituminosa, Sisymbrium irio leaves were sequentially separated
with a nonpolar solvent hexane, while the hydrophilic fractions of the studied plants were separated
with polar solvents ethanol and water. The activity of α-amylase inhibition was carried out by using α-amylase
porcine pancreatic enzyme and 3,5-dinitrosalicylic acid (DNSA) method as well as by using Acarbose as a positive
control. Among the studied plant’s hydrophilic fractions, C. iberica and C. endivia have the highest porcine
pancreatic α-amylase inhibitory effect with an IC50 value of 12.33 μg/mL and 9.96 μg/mL, respectively. In addition,
among the studied plant’s lipophilic fractions, S. irio and A. palaestinum have the highest porcine pancreatic
α-amylase inhibitory effect with an IC50 value of 7.72 μg/mL and 25.3 μg/mL, respectively. In fact, these
revealed results were near the values of Acarbose. The hydrophilic fractions of M. sylvestris and the lipophilic
fractions of P. major plants exhibit remarkable α-amylase inhibitory activity. Hence, these leaves have a potential
for use as regular supplements also; further investigations are required to isolate pure pharmacological
molecules and to design suitable pharmaceutical dosage forms with anti-diabetic activity.

العنوان

J Complement Integr Med.

الناشر

De Gruyter

بلد الناشر

ألمانيا

Indexing

Scopus

معامل التأثير

None

نوع المنشور

Both (Printed and Online)

المجلد

19

السنة

2019

الصفحات

271-279